Electrophoretic variation of isozymes in plumules of rice (Oryza saliva L.) - a key to the identification of 76 alleles at 24 loci

Glaszmann, J. C. ; de los Reyes, B. G. ; Khush, G. S. (1988) Electrophoretic variation of isozymes in plumules of rice (Oryza saliva L.) - a key to the identification of 76 alleles at 24 loci International Rice Research Institute Research Paper Series (IRRI) (134). pp. 1-15. ISSN 0115-3862

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Abstract

A simple and efficient method has been developed for studying isozyme variation in rice. It involves starch gel electrophoresis of crude rice plumule extracts, followed by staining of 13 enzymes. It permits monitoring the variation at 24 polymorphic loci distributed on at least 8 chromosomes. Technical procedures are described, and the zymograms obtained from materials containing all known alleles are shown. A total of 76 alleles can be readily identified.

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Source:Copyright of this article belongs to International Rice Research Institute, Manila, Philippines.
ID Code:83787
Deposited On:22 Feb 2012 12:57
Last Modified:19 May 2016 00:30

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