A high frequency of human papillomavirus DNA sequences in cervical carcinomas of indian women as revealed by southern blot hybridization and polymerase chain reaction

Das, B. C. ; Sharma, J. K. ; Gopalkrishna, V. ; Das, D. K. ; Singh, V. ; Gissmann, L. ; Zur Hausen, H. ; Luthra, Usha K. (1992) A high frequency of human papillomavirus DNA sequences in cervical carcinomas of indian women as revealed by southern blot hybridization and polymerase chain reaction Journal of Medical Virology, 36 (4). pp. 239-245. ISSN 0146-6615

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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/jmv.189...

Related URL: http://dx.doi.org/10.1002/jmv.1890360402

Abstract

Ninety-six colposcopical ly directed biopsies from squamous epithelial carcinoma of the uterine cervix and 22 age-matched normal control biopsy specimens were examined by both Southern blot hybridization and polymerase chain reaction (PCR) for the presence of different human papillomavirus (HPV) DNA types. Cancer of the uterine cervix, which is the most common malignant disease in Indian women, showed a high frequency (98%) of HPV as compared to those reported from other parts of the world. HPV type 16 was found to be the dominant (64%) type while the frequency of HPV type 18 was verylow (3%). On individual typing of HPV, no biopsy was found to contain any other known HPV types under stringent conditions of hybridization except a single case of HPV type 11. Only one case of double infection with HPV types 16 and 18 was recorded. Under low stringency conditions of hybridization with a mixed probe of HPV types 16 and 18, 29 additional biopsies were found to be positive. Southern blot hybridization alone detected HPV DNA in 92% of the cases but none in the controls. By PCR, six (6.25%) more cases and four (18.18%) healthy women were found to be positive for HPVs. Analysis of the physical state of HPV 16 indicated integration in about 70% of carcinoma cases while 30% of them were in episomal form. The findings suggest that infection with HPV is an important etiologic factor for the development of cervical cancer, that a number of such tumours may arise without HPV infection, and that integration of the viral DNA into host genome is not always essential for malignant progression. Furthermore, the use of PCR provides an effective complementation of Southern blot hybridization for meaningful epidemiological studies of HPV infection.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons, Inc.
Keywords:Human Papillomavirus; Cervical Cancer; Southern Blot Hybridization; Polymerase Chain Reaction; Physical State of HPV; Oligoprimers; Two Dimensional Gel Electrophoresis
ID Code:8322
Deposited On:26 Oct 2010 11:46
Last Modified:30 May 2011 08:14

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