Chauhan, V. S. ; Rao, M. V. R. ; Atreyi, M. ; Kumar, Satish ; Kumar, G. Suresh (1984) On the interaction of the synthetic 53-58 fragment of lac repressor with DNA International Journal of Biological Macromolecules, 6 (6). pp. 353-354. ISSN 0141-8130
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/014181...
Related URL: http://dx.doi.org/10.1016/0141-8130(84)90022-9
Abstract
The hexapeptide fragment 53-58 of the lac repressor protein (Ala-Gln-Gln-Leu-Ala-Gly) was synthesized. Circular dichroic spectrum of the peptide has negative bands at 195 and 225 nm (weak). The hexapeptide, which has no basic residues, stabilized the AT rich Clostridium perfringens DNA, but had no effect, significantly, on the melting profile of either poly dA - poly dT or poly d(A-T)·poly d(A-T). The melting temperature of Micrococcus lysodeikticus DNA with a high GC content, was unaffected by the peptide.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Lac Repressor Protein; Hexapeptide; poly dA-poly dT |
| ID Code: | 8260 |
| Deposited On: | 26 Oct 2010 11:59 |
| Last Modified: | 30 May 2011 11:51 |
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