Prasanna Kumar, K. ; Chatterji, D. (1988) An improved method for the purification of DNA-dependent RNA polymerase from Escherichia coli Journal of Biochemical and Biophysical Methods, 15 (5). pp. 235-240. ISSN 0165-022X
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0165-022X(88)90010-3
Abstract
DNA-dependent RNA polymerase from Escherichia coli was purified further by elution through heparin-Sepharose CL-6B column after the enzyme was obtained, partially purified, using Burgess and Jendrisak's method [(1975)Biochemistry 14, 4634] The total yield of the pure protein was 10 mg from 50 g of E. coli cells. The method was found to be very reproducible and convenient. The enzyme preparation had 60% active molecules and the elongation rate of RNA synthesis by this enzyme was measured to be 11 bases/g over δ D111 T7 DNA.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Purification; RNA polymerase, E. coli.; Heparin-Sepharose column |
| ID Code: | 82217 |
| Deposited On: | 10 Feb 2012 04:39 |
| Last Modified: | 10 Feb 2012 04:39 |
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