Effector T cells immune reactivity among patients with acute hepatitis

Husain, M. M. ; Aggarwal, R. ; Kumar, D. ; Jameel, S. ; Naik, S. (2011) Effector T cells immune reactivity among patients with acute hepatitis Journal of Viral Hepatitis, 18 (10). e603-e608. ISSN 1352-0504

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-...

Related URL: http://dx.doi.org/10.1111/j.1365-2893.2011.01489.x


Hepatitis E virus (HEV) is a leading cause of acute viral hepatitis in several developing countries. Information on cellular immune responses during acute hepatitis E is limited. We therefore studied peripheral blood mononuclear cells (PBMCs) from patients with acute hepatitis E and healthy adult subjects who lacked anti-HEV antibodies for enumeration of various T-cell subsets using flow cytometry and to assess HEV-specific T effector cell responses using interferon-gamma ELISPOT assays. The patients showed increased numbers of CD8+ cells and CD4+CD8+ cells compared with healthy controls. In addition, the proportion of PBMCs that produced interferon-gamma in response to recombinant HEV open reading frame (ORF) 2 and ORF 3 proteins were found to be higher in patients than in healthy controls. Using pools of 15-mer overlapping peptides corresponding to these recombinant proteins, the immunodominant regions in these proteins for interferon-gamma-producing cells were mapped to regions corresponding to amino acids 181-249 and 301-489 of HEV ORF2 protein. These data provide evidence for the activation of effector T cells during acute hepatitis E. These responses may play a role in viral clearance from the host in patients with HEV infection.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
Keywords:CD4 cells; CD4+CD8+ Double-positive Cells; CD8 Cells; Cellular Immune Responses; ELISPOT Assays; Gamma-interferon; Hepatitis E; T Cells
ID Code:81931
Deposited On:08 Feb 2012 15:30
Last Modified:08 Feb 2012 15:30

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