An improved technique for isolation of environmental vibrio cholerae with epidemic potential: monitoring the emergence of a multiple-antibiotic-resistant epidemic strain in Bangladesh

Faruque, Shah M. ; Johirul Islam, M. ; Ahmad, Qazi Shafi ; Biswas, Kuntal ; Faruque, A. S. G. ; Balakrish Nair, G. ; Bradley Sack, R. ; Sack, David A. ; Mekalanos , John J. (2006) An improved technique for isolation of environmental vibrio cholerae with epidemic potential: monitoring the emergence of a multiple-antibiotic-resistant epidemic strain in Bangladesh The Journal of Infectious Diseases, 193 (7). pp. 1029-1036. ISSN 0022-1899

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Official URL: http://jid.oxfordjournals.org/content/193/7/1029.s...

Related URL: http://dx.doi.org/10.1086/500953

Abstract

Predicting cholera epidemics through monitoring the environment for the presence of pathogenic Vibrio cholerae is complicated by the presence in water of a large number of mostly nonpathogenic V. cholerae strains. V. cholerae strains causing recent cholera epidemics in Bangladesh carry the sulfamethoxazole-trimethoprim (SXT) element, which encodes resistance to several antibiotics. Here, we show that the use of a culture medium containing streptomycin, sulfamethoxazole, and trimethoprim (the antibiotic selection technique [AST]) can significantly enhance the isolation of environmental V. cholerae O1 with epidemic potential (P<.001). The AST was also used to monitor the recent emergence and spread of a new multiple-antibiotic-resistant strain of V. cholerae in Bangladesh. The results of this study support the hypothesis that pre-epidemic amplification of pathogenic V. cholerae occurs in the human host and leads to the start of an epidemic cycle dominated by a single clone of V. cholerae that spreads rapidly through environmental waters.

Item Type:Article
Source:Copyright of this article belongs to University of Chicago Press.
ID Code:80636
Deposited On:01 Feb 2012 12:49
Last Modified:01 Feb 2012 12:49

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