On the disulfide-linker strategy for designing efficacious cationic transfection lipids: an unexpected transfection profile

Kumara, Valluripalli Vinod ; Chaudhuri, Arabinda (2004) On the disulfide-linker strategy for designing efficacious cationic transfection lipids: an unexpected transfection profile FEBS Letters, 571 (1-3). pp. 205-211. ISSN 0014-5793

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00145...

Related URL: http://dx.doi.org/10.1016/j.febslet.2004.06.058

Abstract

Herein, employing a previously reported disulfide-linker strategy, we have designed and synthesized a novel cationic lipid 2 with a disulfide-linker and its non-disulfide control analog lipid 1. The relative efficacies of lipids 1 and 2 in transfecting CHO, COS-1 and MCF-7 cells were measured using both reporter gene and whole cell histochemical staining assays. In stark contrast to the expectation based on the disulfide-linker strategy, the control non-disulfide cationic lipid 1 showed phenomenally superior in vitro transfection efficacies to its essentially transfection incompetent disulfide counterpart lipid 2. Results in DNase I protection experiments and the electrophoretic gel patterns in the presence of glutathione, taken together, are consistent with the notion that the success of the disulfide-linker strategy may depend more critically on the DNase I sensitivity of the lipoplexes than on the efficient DNA release induced by intracellular glutathione pool.

Item Type:Article
Source:Copyright of this article belongs to Federation of European Biochemical Societies.
Keywords:Disulfide-linker; Intracellular Reduction; DNA Transfection; Lipoplexes; Lipofection; Transgene Expression
ID Code:8047
Deposited On:25 Oct 2010 10:04
Last Modified:16 May 2016 18:07

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