Isolation and molecular characterization of toxigenic Vibrio parahaemolyticus from the Kii Channel, Japan

Mahmud, Zahid Hayat ; Kassu, Afework ; Mohammad, Alizadeh ; Yamato, Masayuki ; Bhuiyanb, N. A. ; Balakrish Nair, G. ; Ota, Fusao (2006) Isolation and molecular characterization of toxigenic Vibrio parahaemolyticus from the Kii Channel, Japan Microbiological Research, 161 (1). pp. 25-37. ISSN 0944-5013

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/j.micres.2005.04.005

Abstract

Studies were conducted on the ecology of potentially pathogenic Vibrio parahaemolyticus in three coastal areas of Kii Channel, Tokushima, Japan. Seawater and seaweed samples were collected seasonally between June 2003 and May 2004. Total and toxigenic strains of V. parahaemolyticus were isolated using most probable number culture and colony blot hybridization. Toxigenic strains were serotyped and further characterized by random amplified polymorphic DNA (RAPD) and ribotyping. Six thousand strains of V. parahaemolyticus were isolated and 18 were found positive for tdh. V. parahaemolyticus were detected in all samples during summer and autumn, and from some samples during winter and spring. Among the toxigenic strains seven serotypes, five ribotypes and RAPD patterns were observed. Seven strains belonged to O3:K6 clone with identical ribotypes and RAPD patterns to that of a pandemic reference strain. The presence of toxigenic V. parahaemolyticus with pandemic potential might indicate a human health risk due to consumption of marine food sources.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Vibrio Parahaemolyticus; Ribotyping; RAPD; Seawater; Seaweed
ID Code:80413
Deposited On:01 Feb 2012 10:21
Last Modified:01 Feb 2012 10:21

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