NMR recipe for sequencing short DNA fragments

Ajaykumar, R. ; Majumdar, K. ; Hosur, R. V. (1990) NMR recipe for sequencing short DNA fragments Current science, 59 (23). pp. 1256-1259. ISSN 0011-3891

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Official URL: http://www.ias.ac.in/j_archive/currsci/59/23/1256-...


A new recipe has been described for determination of the base sequence in short DNA segments by two - dimensional NMR spectroscopy. The recipe is based on (i) A,T,G,C-distinguishing criteria obtained by analysis of chemical shifts of the non-exchangeable protons and (ii) cross-peak patterns in two-dimensional COSY and NOESY spectra. The base H8 and sugar H2" chemical shifts have been found to be characteristically dependent on the base type to which they belong and the patterns of H8-H2" cross-peaks in NOESY allow determination of sequence of bases in DNA segments.

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