Conformational changes in the nitrogenase complex in vivo by preincubation under acetylene

Apte, S. K. ; David, K. A. V. ; Thomas, Joseph (1978) Conformational changes in the nitrogenase complex in vivo by preincubation under acetylene Biochemical and Biophysical Research Communications, 83 (3). pp. 1157-1163. ISSN 0006-291X

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000629...

Related URL: http://dx.doi.org/10.1016/0006-291X(78)91516-4

Abstract

Preincubation of Anabaena L-31 under acetylene results in conformational changes in the nitrogenase enzymecomplex. In particular, the site common to both nitrogen and acetylene is modified such that the affinity towards acetylene increases considerably and there is a corresponding decrease in the affinity towards nitrogen. The site for azide remains unaltered. The binding site for carbon-monoxide (CO) also undergoes change as revealed by the remarkable decrease in the affinity for CO. The results explain the enhancement in acetylene reduction upon preincubation under acetylene.

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