Endometrium - an extragonadal source of inhibin

Garde, S. V. ; Sheth, A. R. ; Zaveri, B. J. ; Shah, J. ; Hinduja, I. (1991) Endometrium - an extragonadal source of inhibin Indian Journal of Experimental Biology, 29 (10). pp. 889-896. ISSN 0019-5189

Full text not available from this repository.

Official URL: http://www.niscair.res.in/ScienceCommunication/Res...


Using polyclonal antibodies generated against human seminal plasma inhibin (10.5 KDa), immunocytochemical localization was carried out in paraffin embedded tissue sections of human endometrial biopsies obtained at various phases of the menstrual cycle. A positive reaction which indicated the presence of inhibin was characterized by the presence of golden yellow or brown colour in the cytoplasm of epithelial cells that formed the glands as well as the luminal lining. The stromal cells however, showed negative staining. In early proliferative phase, the endometrial glands exhibited weak positive staining for inhibin which gradually increased and was intense in late follicular and early secretory phases. The intensity of the staining although was not diminished in the glandular epithelium in the mid as well as late secretory phases, the number of cells showing positive staining appeared to be reduced. Incubation of endometrial biopsies in vitro with labelled amino acid and immunoprecipitation of newly synthesized protein with specific antibodies to inhibin indicated that endometrium is capable of de novo synthesizing inhibin. The above results suggest that endometrium is an extra ovarian source of inhibin and the possible role of endometrial peptide in sperm fertilizing capabilities as well as in pre and post implantation events is suggested.

Item Type:Article
Source:Copyright of this article belongs to National Institute of Science Communication and Information Resources.
ID Code:78225
Deposited On:18 Jan 2012 08:16
Last Modified:18 Jan 2012 08:16

Repository Staff Only: item control page