Shahani, Savitri K. ; Bordekar, Arvind D. ; Shah, Girish V. ; Sheth, Anil R. ; Rao, Shanta S. (1978) Raising of antisera to protein hormones without using Freund's adjuvant Journal of Immunological Methods, 23 (1-2). pp. 91-97. ISSN 0022-1759
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0022-1759(78)90112-6
Abstract
A new method has been developed to produce antiserum to protein hormones without the use of Freund's complete adjuvant. Antiserum to human chorionic gonadotropin (hCG) was elicited by injecting rabbits with 6 subcutaneous injections of sheep red blood cells)SRBC) coated with hCG. The antiserum produced was observed to be both serologically and biologically active. A small quantity of the hormone (15-20 IU/injection) sufficed to produce hyperimmune sera. This could ensure an overall economy if large amounts of the antisera are required for clinical and diagnostic purposes.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 78025 |
| Deposited On: | 16 Jan 2012 12:38 |
| Last Modified: | 16 Jan 2012 12:38 |
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