Pectins as mediators of wall porosity in soybean cells

Baron-Epel, Orna ; Gharyal, Paramjit K. ; Schindler, Melvin (1988) Pectins as mediators of wall porosity in soybean cells Planta, 175 (3). pp. 389-395. ISSN 0032-0935

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The non-invasive technique of fluorescence redistribution after photobleaching was employed on soybean (Glycine max (L.) Merr.) root cells grown in suspension culture to examine macromolecular transport across plant cell walls. Using both fluorescently derivatized dextrans and proteins of graded size, a functional range of diameters for putative trans-wall channels was determined to be 6.6-8.6 nm. A mild treatment with pectinase apparently enlarged the channels, without adversely affecting cell viability, enabling significantly larger molecules to pass through the wall. Treatment of the cells with cellulysin or protease did not have this enlargement effect. It appears that the organization of pectic substances is a major control element in defining the sieving properties of the wall.

Item Type:Article
Source:Copyright of this article belongs to Springer.
Keywords:Cell Culture (Wall Porosity); Fluorescence Redistribution; Glycine (Cell Wall); Pectin Organization
ID Code:77765
Deposited On:14 Jan 2012 12:36
Last Modified:14 Jan 2012 12:36

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