Gupta, R. S. ; Singh, U. N. (1972) Initiation of transcription and the sequential synthesis of 16-S and 23-S ribosomal RNA in Escherichia coli. Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 277 (3). pp. 567-575. ISSN 0005-2787
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0005-2787(72)90100-1
Abstract
RNA synthesis in Escherichia coli, preincubated in the presence of rifampicin, on transfer to antibiotic-free medium shows a partial recovery, presumably due to fresh initiations of transcription. Incorporation of [14C]uracil into RNA during this period when chased by unlabelled uracil first appears in 16-S rRNA. Radioactivity in 23-S rRNA is detectable only for labelling periods >40 s. It is concluded that 16-S and 23-S rRNA genes are contiguous; the former being located at the proximal end in reference to a common initiation site. The transcription time for 16-S rRNA inferred from the observed time-difference between the appearance of radioactivity in 16- and 23-S rRNA is found to be ⋍ 35 s.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 77288 |
| Deposited On: | 11 Jan 2012 04:27 |
| Last Modified: | 11 Jan 2012 04:27 |
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