Seshachar, B. R. ; Saxena, D. M. (1968) DNA-dependent RNA synthesis in isolated macronuclei of Blepharisma intermedium (Ciliata) Journal of Eukaryotic Microbiology, 15 (4). pp. 697-700. ISSN 0022-104X
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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1550-...
Related URL: http://dx.doi.org/10.1111/j.1550-7408.1968.tb02197.x
Abstract
Autoradiographic studies on DNA-dependent RNA synthesis in isolated macronuclei of Blepharisma intermedium in vitro are described. Isolated macronuclei incubated in a medium containing RNA precursors incorporated cystidine and uridine into RNA. Uptake of cystidine was greater than that of uridine. Addition of ATP did not appreciably affect the uptake of precursors into RNA. Actinomycin D (5 μg/ml) blocked incorporation of cystidine and uridine. It is concluded that in isolated macronuclei of Blepharisma, RNA precursors are 1st converted into triphosphates and then incorporated into RNA thru DNA-dependent RNA polymerase.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to John Wiley and Sons. |
| ID Code: | 76987 |
| Deposited On: | 09 Jan 2012 08:23 |
| Last Modified: | 09 Jan 2012 08:23 |
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