Anthranilic acid hydroxylase from Tecoma stans

Madhusudanan Nair, P. ; Vaidyanathan, C. S. (1965) Anthranilic acid hydroxylase from Tecoma stans Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation, 110 (3). pp. 521-531. ISSN 0926-6593

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/S0926-6593(65)80065-0

Abstract

A hydroxylase catalysing the conversion of anthranilic acid to 3-hydroxy-anthranilic acid has been isolated from the leaves of Tecoma stans. The enzyme was purified by fractional precipitation with acetone and adsorption on DEAE-cellulose to give about 84-fold increase in specific activity. The optimum ρ H for the reaction was 5.0. Among the compounds tested, only anthranilic acid was effective as substrate. The enzyme was inhibited by concentrations of anthranilic acid above 1 mM. Metal-chelating agents and sulfhydryl compounds produced marked inhibition. The enzyme was activated by Fe3+ and the inhibition with α -α ′-dipyridyl was specifically reversed by Fe3+. The enzyme showed an absolute requirement for tetrahydrofolic acid and reduced nicotinamide coenzymes for activity.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:76940
Deposited On:09 Jan 2012 03:48
Last Modified:09 Jan 2012 03:48

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