Terenius, Olle ; Papanicolaou, Alexie ; Jennie Garbutt, S. ; Eleftherianos, Ioannis ; Huvenne, Hanneke ; Kanginakudru, Sriramana ; Albrechtsen, Merete ; An, Chunju ; Aymeric, Jean-Luc ; Barthel, Andrea ; Bebas, Piotr ; Bitra, Kavita ; Bravo, Alejandra ; Chevalier, Francois ; Derek Collinge, P. ; Cristina Crava, M. ; Ruud de Maagd, A. ; Duvic, Bernard ; Erlandson, Martin ; Faye, Ingrid ; Felfoldi, Gabriella ; Fujiwara, Haruhiko ; Futahashi, Ryo ; Archana Gandhe, S. ; Heather Gatehouse, S. ; Laurence Gatehouse, N. ; Jadwiga Giebultowicz, M. ; Gomez, Isabel ; Cornelis Grimmelikhuijzen, J.P. ; Astrid Groot, T. ; Hauser, Frank ; David Heckel, G. ; Dwayne Hegedus, D. ; Hrycaj, Steven ; Huang, Lihua ; Joe Hull, J. ; Iatrou, Kostas ; Iga, Masatoshi ; Michael Kanost, R. ; Kotwica, Joanna ; Li, Changyou ; Li, Jianghong ; Liu, Jisheng ; Lundmark, Magnus ; Matsumoto, Shogo ; Meyering- Vos, Martina ; Peter Millichap, J. ; Monteiro, Antonia ; Mrinal, Nirotpal ; Niimi, Teruyuki ; Nowara, Daniela ; Ohnishi, Atsushi ; Oostra, Vicencio ; Ozaki, Katsuhisa ; Papakonstantinou, Maria ; Popadic, Aleksandar ; Manchikatla Rajam, V. ; Saenko, Suzanne ; Robert Simpson, M. ; Soberon, Mario ; Michael Strand, R. ; Tomit, Shuichiro ; Toprak, Umut ; Wang, Ping ; Wee, Choon Wei ; Whyard, Steven ; Zhang, Wenqing ; Nagaraju, Javaregowda ; Richard ffrench- Constant, H. ; Herrero, Salvador ; Gordon, Karl ; Swevers, Luc ; Smagghe, Guy (2011) RNA interference in Lepidoptera: an overview of successful and unsuccessful studies and implications for experimental design Journal of Insect Physiology, 57 (2). pp. 231-245. ISSN 0022-1910
Full text not available from this repository.
Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/j.jinsphys.2010.11.006
Abstract
Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity. On the contrary, gene expression in epidermal tissues seems to be most difficult to silence. In addition, gene silencing by feeding dsRNA requires high concentrations for success. Possible causes for the variability of success in RNAi experiments in Lepidoptera are discussed. The review also points to a need to further investigate the mechanism of RNAi in lepidopteran insects and its possible connection to the innate immune response. Our general understanding of RNAi in Lepidoptera will be further aided in the future as our public database at http://insectacentral.org/RNAi will continue to gather information on RNAi experiments.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | RNA Interference; Lepidoptera; Delivery Methods; Tissue Uptake; Gene Function; DsRNA Properties |
| ID Code: | 76668 |
| Deposited On: | 04 Jan 2012 15:20 |
| Last Modified: | 04 Jan 2012 15:20 |
Repository Staff Only: item control page
