Parnaik, V. K. ; Kennady, P. K. (1990) Nuclear transport of proteins translated in vitro from SP6 plasmid-generated mRNAs Molecular and Cellular Biology, 10 (3). pp. 1287-1292. ISSN 0270-7306
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Official URL: http://mcb.asm.org/content/10/3/1287.short
Related URL: http://dx.doi.org/10.1128/?MCB.10.3.1287
Abstract
A sensitive and versatile assay is described for the nuclear transport of 35S-labeled proteins obtained by the in vitro translation of SP6 plasmid-generated mRNAs. A specific nuclear accumulation of greater than 20-fold is observed for the transformation-related nuclear proteins, p53 and E1b, and the nuclear enzyme, thymidine kinase, whereas transport of the nonnuclear proteins, dihydrofolate reductase and simian virus 40 small t antigen, is negligible within 30 min.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to American Society for Microbiology. |
| ID Code: | 75318 |
| Deposited On: | 22 Dec 2011 13:04 |
| Last Modified: | 22 Dec 2011 13:04 |
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