1BL.1RS translocation in some Indian bread wheat genotypes and strategies for its use in wheat breeding

Kumar, S. ; Kumar, N. ; Balyan, H. S. ; Gupta, P. K. (2003) 1BL.1RS translocation in some Indian bread wheat genotypes and strategies for its use in wheat breeding Caryologia, 56 (1). pp. 23-30. ISSN 0008-7114

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Abstract

In the present study 17 bread wheat genotypes including Chinese Spring were screened to identify 1BL.1RS translocation (T1BL.1RS). Both molecular approaches and karyotype analysis were used. In 8 of the 17 bread wheat genotypes, the translocation 1BL.1RS was identified using GISH (genomic in situ hybridization) involving total rye genomic DNA as a probe, which allowed identification of a rye chromosome arm carrying a relatively small satellite that is characteristic of 1RS. The microsatellite (SSR) markers specific for 1BS successfully amplified only in 9 genotypes including Chinese Spring, and gave no PCR product in the remaining 8 genotypes, thus confirming the loss of 1BS arm in these 8 genotypes. The 1BL specific microsatellite (SSR) markers, on the other hand, amplified successfully in all the 17 genotypes. Further, in all the above 8 genotypes, instead of normal four prominent satellites, only two prominent satellites were observed at somatic metaphase confirming the replacement of 1BS with 1RS, resulting in T1BL.1RS in these genotypes. The 8 genotypes that carried T1BL.1RS included the following: PBW373, PBW343, PBW175, UP2338, UP2425, UP2418, UP2382 and CPAN3004. These varieties may be considered as a useful germplasm resource for wheat breeding.

Item Type:Article
Source:Copyright of this article belongs to University of Florence / Firenze, Italy.
Keywords:GISH; STMS; Translocation; Wheat
ID Code:74969
Deposited On:20 Dec 2011 05:24
Last Modified:18 May 2016 19:11

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