The Bacteriophage λ DNA replication protein P inhibits the oriC DNA-and ATP-binding functions of the DNA replication initiator protein DnaA of escherichia coli

Datta, Indrani ; Sau, Subrata ; Sil, Alok Kumar ; Mandal, Nitai C. (2005) The Bacteriophage λ DNA replication protein P inhibits the oriC DNA-and ATP-binding functions of the DNA replication initiator protein DnaA of escherichia coli Journal of Biochemistry and Molecular Biology, 38 (1). pp. 97-103. ISSN 1225-8687

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Abstract

Under the condition of expression of λ P protein at lethal level, the oriC DNA-binding activity is significantly affected in wild-type E. coli but not in the rpl mutant. In purified system, the λ P protein inhibits the binding of both oriC DNA and ATP to the wild-type DnaA protein but not to the rpl DnaA protein. We conclude that the λ P protein inhibits the binding of oriC DNA and ATP to the wild-type DnaA protein, which causes the inhibition of host DNA synthesis initiation that ultimately leads to bacterial death. A possible beneficial effect of this interaction of λ P protein with E. coli DNA initiator protein DnaA for phage DNA replication has been proposed.

Item Type:Article
Source:Copyright of this article belongs to Springer.
Keywords:dnaA Gene of E. Coli; λ P Gene; Oric DNA; λ P-DnaA Interaction; λ P Gene Lethality
ID Code:74578
Deposited On:17 Dec 2011 10:04
Last Modified:17 Dec 2011 10:04

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