Garg, U. C. ; Rai, N. ; Singh, Y. ; Dhaunsi, G. S. ; Sidhu, G. S. ; Ganguly, N. K. ; Bhatnagar, R. (1988) A spectrophotometric method for calmodulin assay Biotechniques, 6 (4). pp. 294-296. ISSN 0736-6205
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Abstract
The activity of calmodulin as an activator of cAMP phosphodiesterase was assayed. AMP was hydrolyzed by 5'-nucleotidase, and the adenosine formed was measured by both liquid scintillation counting and spectrophotometry at 265 nm. Calmodulin activities measured by the two methods were equivalent, indicating that spectrophotometric assay of calmodulin can be used in place of the isotopic method.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Informa plc. |
| ID Code: | 74262 |
| Deposited On: | 09 Dec 2011 05:31 |
| Last Modified: | 13 Jul 2012 12:04 |
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