Expression of cholera toxin B subunit in transgenic tomato plants

Jani, Dewal ; Meena, Laxman Singh ; Rizwan-ul-Haq, Quazi Mohammad ; Singh, Yogendra ; Sharma, Arun K. ; Tyagi, Akhilesh K. (2002) Expression of cholera toxin B subunit in transgenic tomato plants Transgenic Research, 11 (5). pp. 447-454. ISSN 0962-8819

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Cholera toxin, secreted by Vibrio cholerae, consists of A and B subunits. The latter binds to GM1-ganglioside receptors as a pentamer (55 kDa). Tomato plants were transformed with the gene encoding cholera toxin B subunit (ctxB) along with an endoplasmic reticulum retention signal (SEKDEL) under the control of the CaMV 35S promoter via Agrobacterium-mediated transformation. PCR and Southern analysis confirmed the presence of the ctxB gene in transformed tomato plants. Northern analysis showed the presence of the ctxB-specific transcript. Immunoblot assays of the plant-derived protein extract showed the presence of cholera toxin subunit B (CTB) with mobility similar to purified CTB from V. cholerae. Both tomato leaves and fruits expressed CTB at levels up to 0.02 and 0.04% of total soluble protein, respectively. The GM1-ELISA showed that the plant-derived CTB bound specifically to GM1-ganglioside receptor, suggesting that it retained its native pentameric form. This study forms a basis for exploring the utility of CTB to develop tomato-based edible vaccines against cholera.

Item Type:Article
Source:Copyright of this article belongs to Springer.
Keywords:Cholera Toxin B Subunit; Edible Vaccine; Gene Expression; GM1-ELISA; Transgenic Plants
ID Code:74248
Deposited On:09 Dec 2011 05:33
Last Modified:21 Jul 2012 14:48

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