Grover, A. ; Tyagi, A. ; Koundal, K. R. ; Sharma, R. P. (1996) Isolation and subcloning of chitinase clone from Chickpea Genomic Library Indian Journal of Experimental Biology, 34 (6). pp. 594-596. ISSN 0019-5189
Full text not available from this repository.
Abstract
Chickpea genomic library constructed earlier in phage lambda (EMBL-3) was screened for the presence of chitinase clone using tobacco chitinase cDNA as a probe. Positive clones obtained by primary screening of plaques (2 × 10(6)) were ascertained by secondary and tertiary screening. Presence of chitinase insert in the positive clones obtained, was further confirmed by restricting phage DNA with Sal I and then doing southern with tobacco chitinase. The insert band was eluted out and subcloned in puc 19 plasmid.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to National Institute of Science Communication and Information Resources. |
| ID Code: | 74069 |
| Deposited On: | 08 Dec 2011 12:17 |
| Last Modified: | 08 Dec 2011 12:17 |
Repository Staff Only: item control page
