Construction of Chickpea Genomic Library

Koundal, K. R. ; Mehta, S. L. ; Sharma, R. P. (1989) Construction of Chickpea Genomic Library Indian Journal of Experimental Biology, 27 (10). pp. 858-860. ISSN 0019-5189

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Abstract

For construction of chickpea genomic library, DNA was isolated, purified on CsCl gradient and size fractionated into 15-20 Kb fragments after restriction with Sau 3A. These fragments were ligated to phage lambda (EMBL-3) vector and the recombinant molecules packaged in vitro into viable phage particles. The recombinant phages were obtained as phages on a P2 lysogen of E. coli (Spi- selection) and amplified to establish a permanent library. This is the first report of the construction of chickpea genomic library.

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