Thomas, G. ; Vasavada, H. A. ; Sachariah, E. ; Padayatty, J. D. (1983) Cloning of rice DNA & identification of the cloned H2A, H2B & H4 histone genes Indian Journal of Biochemistry & Biophysics, 20 (1). pp. 8-15. ISSN 0301-1208
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Abstract
DNA isolated from 48-h germinated rice embryos and cut with restriction endonuclease BamHI was inserted at the BamHI site in plasmid pBR322 and cloned in Escherichia coli HB101. Recombinantion of DNA-contg. clones were selected by their resistance to ampicillin and sensitivity to tetracycline and identified by colony hybridization to homologous probes comprising 32P-labeled RNA from 18-h germinated rice embryos. Two clones contg. genes for 9-12 S poly(A)- RNA, which was enriched in histone messengers, were identified. One of the clones contained an insert of 6.64 kilobases. RNAs that hybridized with this insert were translated into H2A4, 4H2B, and H4 histones. Thus, the inserted DNA contains these genes.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to National Institute of Science Communication and Information Resources.. |
| ID Code: | 73693 |
| Deposited On: | 07 Dec 2011 05:42 |
| Last Modified: | 07 Dec 2011 05:42 |
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