Nataraju, B. ; Sivaprasad, V. ; Datta, R. K. ; Gupta, S. K. ; Shamim, Mohd (1994) Colloidal textile dye-based dipstick immunoassay for the detection of nuclear polyhedrosis virus (BmNPV) of silkworm, Bombyx mori L. Journal of Invertebrate Pathology, 63 (2). pp. 135-139. ISSN 0022-2011
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1006/jipa.1994.1026
Abstract
A colloidal textile dye-based dipstick immunoassay was developed for the detection of Bombyx mori nuclear polyhedrosis virus (BmNPV). The textile dye-based dipstick immunoassay favorably compares with the enzyme-based dipstick assay with regard to simplicity, sensitivity, storage, and cost efficiency. Dipstick textile blue D2R dye (Aλmax = 620), sensitized with 500 μg/ml of affinity purified anti-BmNPV IgG, could detect 10 ng/ml of antigen by forming a clear blue dot in 30 min. The sensitized dye was observed to be stable for a 3-month period at 4·C. The colloidal dye-based dipstick immunoassay method provides a simple and sensitive test for the detection of BmNPV in silkworms.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Nuclear Polyhedrosis Virus; Bombyx mori; Colloidal Textile Dye; Nitrocellulose Membrane; Dipstick Immunoassay |
| ID Code: | 72160 |
| Deposited On: | 28 Nov 2011 06:09 |
| Last Modified: | 28 Nov 2011 06:09 |
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