Somatic mutation of immunoglobulin lambda chains: a segment of the major intron hypermutates as much as the complementarity-determining regions

González-Fernández, A. ; Gupta, S. K. ; Pannell, R. ; Neuberger, M. S. ; Milstein, C. (1994) Somatic mutation of immunoglobulin lambda chains: a segment of the major intron hypermutates as much as the complementarity-determining regions PNAS, 91 (26). pp. 12614-12618. ISSN 0027-8424

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Official URL: http://www.pnas.org/content/91/26/12614.short

Abstract

The rate and nature of hypermutation of immunoglobulin genes are of prime importance in the affinity maturation of antibodies. Although a considerable body of information has been gathered for κ light chains, there is much less data for λ chains. We have derived a large data base of somatic mutants of mouse λ 1 light chains from Peyer's patches germinal center B cells. The endogenous λ 1 genes mutate at a rate comparable to that previously found for a κ transgene (V κ ox1). There are intrinsic hot spots of mutation common to both in-frame and out-of-frame rearrangements; these hot spots cluster in hypermutating domains. In contrast to the pattern seen for V κ Ox1, the hot spot clusters are found not only in complementarity-determining region (CDR)1 but also in CDR2 and CDR3; mutations also cluster in the joining/constant region intron. The differences between the pattern of mutations in V κ Ox1 and λ 1 light chains are discussed.

Item Type:Article
Source:Copyright of this article belongs to National Academy of Sciences.
ID Code:72158
Deposited On:28 Nov 2011 06:09
Last Modified:28 Nov 2011 06:09

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