Gupta, S. K. ; Kaul, Renuka ; Rajalakshmi, S. ; Sahai, P. ; Yurewicz, E. C. ; Sacco, A. G. (1994) Immunoreactivity with native zona pellucida of antibodies against a 19 amino acid synthetic peptide corresponding to human ZP3 Journal of Reproductive Immunology, 27 (3). pp. 241-247. ISSN 0165-0378
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0165-0378(94)90007-8
Abstract
Immunization of female Syrian hamsters with a 19mer-synthetic peptide corresponding to amino acid sequence 323-341 of human ZP3 and coupled to diphtheria toxoid (DT) led to generation of antibodies against both the peptide as well as DT. Antisera showed positive reaction in ELISA with solubilized isolated zona pellucida (SIZP) from human oocytes. In an indirect immunofluorescence assay, the anti-peptide antibodies bound to zona pellucida of human and bonnet monkey but failed to recognize that of mouse, rabbit, hamster and dog. These studies will help in designing a synthetic peptide based ZP immunocontraceptive vaccine for human application.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Mammalian Oocyte; Synthetic Peptide; ELISA; Indirect Immunofluorescence |
| ID Code: | 72157 |
| Deposited On: | 28 Nov 2011 06:09 |
| Last Modified: | 28 Nov 2011 06:09 |
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