Organization and dynamics of N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-labeled lipids: a fluorescence approach

Mukherjee, Soumi ; Raghuraman, H. ; Dasgupta, Sudeshna ; Chattopadhyay, Amitabha (2004) Organization and dynamics of N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-labeled lipids: a fluorescence approach Chemistry and Physics of Lipids, 127 (1). pp. 91-101. ISSN 0009-3084

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00093...

Related URL: http://dx.doi.org/10.1016/j.chemphyslip.2003.09.004

Abstract

Lipids that are labeled with the NBD (7-nitrobenz-2-oxa-1,3-diazol-4-yl) group are widely used as fluorescent analogues of native lipids in biological and model membranes to monitor a variety of processes. NBD-labeled lipids have previously been used to monitor the organization and dynamics of molecular assemblies such as membranes, micelles and reverse micelles utilizing the wavelength-selective fluorescence approach. In this paper, we have characterized the organization and dynamics of various NBD-labeled lipids using red edge excitation shift (REES) and other fluorescence approaches which include analysis of membrane penetration depths of the NBD group using the parallax method. We show here that the environment and location experienced by the NBD group of the NBD-labeled lipids could depend on the ionization state of the lipid. This could have potentially important implications in future studies involving NBD-labeled lipids as tracers in a cellular context.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:NBD-labeled Lipids; REES; Membrane Penetration Depth; Fluorescence Polarization; Ionization State; Fluorescence Lifetime
ID Code:7110
Deposited On:25 Oct 2010 12:34
Last Modified:16 May 2016 17:21

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