Mohapatra, T. ; Sharma, R. P. ; Chopra, V. L. (1992) Cloning and use of low copy sequence genomic DNA for RFLP analysis of somaclones in mustard Brassica juncea L. Current Science, 62 (6). pp. 482-484. ISSN 0011-3891
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Abstract
Brassica juncea (L.) Czern and Coss genomic DNA fragments of 0.5 to 2.5 kb size, obtained by Pst I digestion were cloned into the pUC18 plasmid vector. Screening of the partial library by dot blot hybridization using labelled total genomic DNA as probe revealed that about 45% of the clones carry low copy number DNA sequences. Using some of these clones as probes, DNA polymorphism could be detected among somaclones of B. juncea cv. Varuna.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Current Science Association |
| ID Code: | 70860 |
| Deposited On: | 02 Jul 2012 12:53 |
| Last Modified: | 18 May 2016 16:48 |
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