Agrobacterium-mediated genetic transformation of chickpea Cicer-arietinum

Mohapatra, T. ; Sharma , R. P. (1991) Agrobacterium-mediated genetic transformation of chickpea Cicer-arietinum Indian Journal of Experimental Biology, 29 (8). pp. 758-761. ISSN 0019-5189

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In leaf and stem explants of chickpea, wild type strains of Agrobacteria were able to induce tumors. These tumors were capable of phytohormone independent growth. A supervirulent strain A281 was found to be most effective. Thus, using an agrobacterium R1601, which carries genes conferring supervirulent phenotype along with a plant selectable marker gene (npt II), transformed calli of chickpea were selected in the presence of 100 micrograms/ml level of kanamycin. Molecular analyses of genomic DNA from transformed calli confirmed the integration of the marker gene into chickpea genome.

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