Membrane organization and dynamics of the G-protein-coupled serotonin1A receptor monitored using fluorescence-based approaches

Pucadyil, Thomas J. ; Kalipatnapu, Shanti ; Chattopadhyay, Amitabha (2005) Membrane organization and dynamics of the G-protein-coupled serotonin1A receptor monitored using fluorescence-based approaches Journal of Fluorescence, 15 (5). pp. 785-796. ISSN 1053-0509

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Official URL: http://www.springerlink.com/content/75640128561jm6...

Related URL: http://dx.doi.org/10.1007/s10895-005-2988-2

Abstract

The G-protein-coupled receptor (GPCR) superfamily represents one of the largest classes of molecules involved in signal transduction across the plasma membrane. Fluorescence-based approaches have provided valuable insights into GPCR functions such as receptor-receptor and receptor-ligand interactions, real-time assessment of signal transduction, receptor dynamics on the plasma membrane, and intracellular trafficking of receptors. This has largely been possible with the use of fluorescent probes such as the green fluorescent protein (GFP) from the jellyfish Aequoria victoria and its variants. We discuss the potential of fluorescence-based approaches in providing novel information on the membrane organization and dynamics of the G-protein-coupled serotonin1A receptor tagged to the enhanced yellow fluorescent protein (EYFP).

Item Type:Article
Source:Copyright of this article belongs to Springer-Verlag.
Keywords:G-protein-coupled Receptor; Serotonin1A Receptors; Green Fluorescent Protein; Enhanced Yellow Fluorescent Protein; Membrane Domains; Cell Surface Dynamics
ID Code:7059
Deposited On:25 Oct 2010 12:43
Last Modified:16 May 2016 17:18

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