Construction and genetic characterization of lambda specialized transducing particles carrying the rglB gene of Escherichia coli K-12

Sozhamannan, Shanmugasundaram ; Dharmalingam, Kuppamuthu (1988) Construction and genetic characterization of lambda specialized transducing particles carrying the rglB gene of Escherichia coli K-12 Current Microbiology, 17 (5). pp. 269-273. ISSN 0343-8651

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Official URL: http://www.springerlink.com/content/x50736l53u4756...

Related URL: http://dx.doi.org/10.1007/BF01571327

Abstract

The rglB gene ofEscherichia coli codes for a restriction activity that cleaves the hydroxymethylated DNA of T2 and T4 phages. Earlier mapping data placed the gene at 98.39 min counterclockwise to the hsd operon. Genetic analysis of the in vivo gene fusions with λ fusion-transducing phages established the location of the rglB gene next to the hsdS gene of the hsdRMS cluster. The methodology used in this study could be extended to similar in vivo physical mapping of closely linked genes.

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