Depth profiling in membranes by fluorescence quenching

Chattopadhyay, Amitabha (1990) Depth profiling in membranes by fluorescence quenching Journal of Biosciences, 15 (3). pp. 143-144. ISSN 0250-5991

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Membrane penetration depth is an important parameter in relation to membrane structure and organization. A methodology has been developed to analyze the membrane penetration depths of fluorescent molecules or groups utilizing differential fluorescence quenching caused by membrane embedded spin-label probes located at different depths. The method involves determination of the parallax in the apparent location of fluorophores, detected when quenching by phospholipids spin-labelled at two different depths is compared. By use of relatively simple algebraic expressions, the method allows calculation of depth in λ . This method has been used to determine the location of fluorophores in NBD-labelled lipids and anthroyloxy-labelled fatty acids in model membranes and of the membrane embedded tryptophan residues in the reconstituted nicotinic acetylcholine receptor.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Fluorescence Quenching; Spin Labelled Phospholipids; Membrane Penetration Depth
ID Code:6985
Deposited On:26 Oct 2010 04:49
Last Modified:16 May 2016 17:14

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