Macrophage protein kinase C: its role in modulating membrane microviscosity and superoxide in leishmanial infection

Chakraborty, Prasanta ; Ghosh, Debjani ; Basu, Mukul K. (2000) Macrophage protein kinase C: its role in modulating membrane microviscosity and superoxide in leishmanial infection The Journal of Biochemistry, 127 (2). pp. 185-190. ISSN 0021-924X

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Official URL: http://jb.oxfordjournals.org/content/127/2/185.sho...

Abstract

Pretreatment of macrophages with PMA, an agonist of PKC, showed diverse effects on degradation and survival of two virulent strains of Leishmania donovani promastigotes. Treatment of macrophages with PMA for 45 min at 37°C generated significant amounts of superoxide anions and reduced the parasite burden of macrophages by up to 48 and 43% when AG83 and GE-1 strains were used for infection. Staurosporine, an inhibitor of PKC, inhibited PMA-dependent killing of the parasites, while tyrphostin AG 126, an inhibitor of protein tyrosine kinase, showed very little effect. Depletion of PKC by prolonged incubation with PMA drastically reduced the superoxide anion generation and increased the uptake and multiplication of the parasites. Finally, to understand the mechanism of higher uptake of the parasites by PKC-depleted macrophages, membrane microviscosity was measured by fluorescence depolarization. Membrane microviscosity was found to be approximately 40% lower in PKC-depleted macrophages than in normal macrophages, indicating the role of membrane fluidity in the infection process. Together, these data suggest PKC activation, superoxide generation, and membrane fluidity are essential factors in the efficient regulation of leishmanial infection.

Item Type:Article
Source:Copyright of this article belongs to Oxford University Press.
Keywords:Leishmania; Donovani; Macrophages; Membrane Microviscosity; Protein Kinase C; Superoxide
ID Code:69514
Deposited On:12 Nov 2011 03:46
Last Modified:12 Nov 2011 03:46

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