In vitro regeneration through organogenesis of Meconopsis simplicifolia - An endangered ornamental species

Sulaiman, I. M. ; Babu, C. R. (1993) In vitro regeneration through organogenesis of Meconopsis simplicifolia - An endangered ornamental species Plant Cell, Tissue and Organ Culture, 34 (3). pp. 295-298. ISSN 0167-6857

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Official URL: http://www.springerlink.com/content/k6k06516544140...

Related URL: http://dx.doi.org/10.1007/BF00029719

Abstract

Meconopsis simplicifolia (D.Don) Walp. could be propagated by induction of adventitious shoots from callus produced on hypocotyl, cotyledon and rosette leaf explants of 4-month-old seedlings. Callus was initiated on agar-solidified Murashige and Skoog medium supplemented with 1 µM kinetin +10 µM α-naphthaleneacetic acid (NAA). Shoots formed when the callus was subcultured on medium supplemented with kinetin or benzyladenine (BA) in combination with NAA, indoleacetic acid, indolebutyric acid or gibberellic acid. Excised shoots were rooted on medium containing auxin with 10 µM NAA producing the best rooting (55%).

Item Type:Article
Source:Copyright of this article belongs to Springer.
Keywords:ex situ Conservation; Himalayan Blue Poppy; Rooting; Shoot Differentiation; Tissue Culture
ID Code:69198
Deposited On:09 Nov 2011 06:12
Last Modified:09 Nov 2011 06:12

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