Role of Ca2+ in induction and secretion of dengue virus-induced cytokines

Dhawan, Ritu ; Khanna, Madhu ; Chaturvedi, U. C. ; Mathur, Asha (1990) Role of Ca2+ in induction and secretion of dengue virus-induced cytokines Journal of Biosciences, 15 (04). pp. 409-416. ISSN 0250-5991

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The present study was undertaken to investigate the role of calcium ions (Ca2+) in the induction and secretion of the dengue type 2 virus induced cytotoxic factor and the cytotoxin. This was done by using calcium channel blocking drugs such as verapamil, nifedipine or diltiazem hydrochloride. The production of cytotoxic factor was significantly reduced by treatment of dengue type 2 virus infected mice with verapamil. Similarly, a dosedependent inhibition of the secretion of cytotoxic factor was observed, when spleen cells of the virus-primed mice were treatedin vitro with the 3 calcium channel blockers. The production of cytotoxin by macrophages was abrogated by pretreatment with calcium channel blockers but had little effect on its secretion as shown by treatment of macrophages with verapamil at 1 h after the induction to later periods up to 18 h. The findings thus show that in the induction of both the cytokines Ca2+ plays a critical role; on the other hand it is required for the secretion of the cytotoxic factor but not for that of the cytotoxin.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Ca2+; Cytokines; Dengue Virus; Cytotoxic Factor; Calcium Channel Blockers
ID Code:6829
Deposited On:26 Oct 2010 05:26
Last Modified:16 May 2016 17:07

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