Induction of hypoglycaemia in Japanese encephalitis virus infection: the role of T lymphocytes

Khanna, N. ; Mathur, A. ; Bharadwaj, M. ; Chaturvedi, U. C. (1997) Induction of hypoglycaemia in Japanese encephalitis virus infection: the role of T lymphocytes Clinical & Experimental Immunology, 107 (02). pp. 282-287. ISSN 0009-9104

[img]
Preview
PDF - Publisher Version
405kB

Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-...

Related URL: http://dx.doi.org/10.1111/j.1365-2249.1997.281-ce1173.x

Abstract

We report here development of hypoglycaemia in the convalescent phase of Japanese encephalitis virus (JEV) infection in mice by the induction of antigen-specific Ly1-2+ T cells in the spleen which mediate hypoglycaemia through the generation of soluble T cell hypoglycaemic factor (TCHF). The TCHF acted in a dose-dependent manner and was found to be trypsin-sensitive and thermolabile. It was purified on Superose-12 high performance liquid chromatography (HPLC) gel filtration column and purified protein migrated as a ~25kD band on SDS-PAGE. The JEV-induced hypoglycaemia coincided with an increased circulating glucagon level, without any alterations in blood insulin and growth hormone concentrations. These effects were mimicked by TCHF. These results indicate that JEV-primed T lymphocytes mediate hypoglycaemia through the production of a soluble hypoglycaemic factor.

Item Type:Article
Source:Copyright of this article belongs to British Society for Immunology.
Keywords:Hypoglycaemia; Japanese Encephalitis Virus; T Cell Factor; T Suppressor Cell; Glucagon
ID Code:6812
Deposited On:26 Oct 2010 05:28
Last Modified:16 May 2016 17:06

Repository Staff Only: item control page