Antibodies specific to a deoxyribotrinucleotide sequence

Jayaraman, K. ; Jacob, T. M. (1980) Antibodies specific to a deoxyribotrinucleotide sequence Molecular Immunology, 17 (6). pp. 777-781. ISSN 0161-5890

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Bovine serum albumin conjugates of two trinucleotides, dpTpTpA and dTpTpAp, were prepared by linking the trinucleotides through their end phosphates by the 'carbodiimide method'. Antibodies were raised in rabbits by injecting the trinucleotide-bovine serum albumin conjugates. Analysis by double diffusion in agar gel, quantitative precipitin reaction and its inhibition by haptens showed clearly the presence of antibodies specific to the whole trinucleotide molecule. The titre of antibodies obtained by the trinucleotide-rabbit serum albumin conjugates with their respective antisera was approximately the same, indicating that linking the trinucleotide through either 5' or 3' phosphate does not have an appreciable effect on the titre of antibodies. The results also demonstrate that the nucleotide(s) away from the carrier protein is more immunodominant than the one linked directly to the protein.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:67976
Deposited On:02 Nov 2011 02:59
Last Modified:02 Nov 2011 02:59

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