Antibodies specific to deoxyguanosine 5'-phosphate

Abstract

Antibodies to deoxyguanosine 5'-phosphate were produced in rabbits using a bovine serum albumin conjugate of the nucleotide. The presence of hapten-specific antibodies in the antisera was shown by double diffusion in agar and the quantitative precipitin reaction. The specificity of the antibodies was determined by measuring the inhibition of the binding of the tritiated hapten to the immunoglobulins by various nucleotides or derivatives as inhibitors. The antibodies were found to be highly specific for the whole nucleotide, the base and sugar being relatively more important than the phosphate. Cross reactivity was observed with other mononucleotides but was of a low order. The antibodies reacted with oligonucleotides containing G residues and also with single-stranded DNA.