A sensitive radioimmunoassay for isepentenyladenosine

Khan, Saleem A. ; Humayun, M. Z. ; Jacob, T. M. (1977) A sensitive radioimmunoassay for isepentenyladenosine Analytical Biochemistry, 83 (2). pp. 632-635. ISSN 0003-2697

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000326...

Related URL: http://dx.doi.org/10.1016/0003-2697(77)90067-7


A sensitive radioimmunoassay for the nucleoside isopentenyladenosine (iA) by using iA-specific antibodies and a nitrocellulose membrane filtration technique is described. The reliability of the method is demonstrated by using specific tRNAs of known structures for the estimation of iA in their digests. This assay can be used to quantitate minute amounts of iA in the presence of a large excess of other nucleosides.

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