Respiratory burst by dengue-virus-induced cytotoxic factor

Misra, Anita ; Mukerjee, Ruma ; Chaturvedi, U. C. (1998) Respiratory burst by dengue-virus-induced cytotoxic factor Medical Principles and Practice, 7 (4). pp. 251-260. ISSN 1011-7571

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This study investigates the induction and release of the superoxide anion (O- 2) and hydrogen peroxide (H2O2) by mouse spleen cells on stimulation with dengue type 2 virus (DV) and a DV-induced cytokine, the cytotoxic factor (mCF). Methods: Normal mice or their spleen cell cultures were inoculated with DV or mCF. At different time periods, the spleen cell supernatants were assayed for the production of O- 2 and H2O2. Results: Inoculation of DV in spleen cell cultures resulted in peak production of O- 2 and H2O2 at 48 and 72 h, respectively, while in DV-infected mouse spleen, the maximum production was on days 7 and 8, which correlated with the appearance of mCF in the milieu. Maximum O- 2 and H2O2 production occurred at 45 min and 1 h after inoculation of 5 µ g of mCF. Pretreatment of mCF with anti-mCF-antiserum inhibited O- 2 and H2O2 release indicating the specificity of the induction by mCF. The enriched subpopulations of macrophages and T cells produced O- 2 and H2O2 and not B cells. Treatment of the cells with superoxide dismutase increased H2O2 release but inhibited O- 2 release and the cytotoxicity in a dose-dependent manner. Conclusion: This showed that O- 2 is responsible for the cytotoxic activity of mCF and not H2O2. In conjunction with our earlier findings that pretreatment with NG-monomethyl-L-arginine inhibited mCF-induced production of NO and the cytotoxicity, it is concluded that the presence of both O- 2 and NO is required for the cytotoxic activity of mCF, thereby indicating a possible role of peroxynitrite.

Item Type:Article
Source:Copyright of this article belongs to Karger Publisher.
Keywords:Dengue Virus; Cytotoxic Factor; Respiratory Burst; Cytotoxicity; Pathogenesis
ID Code:6777
Deposited On:22 Oct 2010 06:44
Last Modified:16 May 2016 17:04

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