Bhatia, Sabhyata ; Singh, Kapil ; Jagannathan, V. ; Lakshmikumaran, Malathi (1993) Organization and sequence analysis of the 5S rRNA genes in Brassica campestris Plant Science, 92 (1). pp. 47-55. ISSN 0168-9452
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0168-9452(93)90065-8
Abstract
The 5S rRNA gene from Brassica campestris has been cloned, sequenced and characterized. The 5S rDNA repeat unit is 495 bp in length. It consists of a highly conserved, 119 bp coding region and a variable non-coding spacer region which separates it from the coding region of the next repeat unit. The 5S rDNA units are organized into clusters of tandem repeats. Sequences responsible for initiation and termination of transcription of the 5S rRNA are present within the repeat unit. Hybridization data with isoschizomers reveals a high amount of 'C' methylation. The size of the repeat unit and the organization of the 5S rRNA genes in other closely related crucifers has been investigated and found to be quite similar to that of B. campestris.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | 5S rDNA; Tandem Repeat; Brassica; Methylation |
| ID Code: | 67649 |
| Deposited On: | 31 Oct 2011 05:34 |
| Last Modified: | 31 Oct 2011 05:34 |
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