Dengue virus-induced cytotoxin releases nitrite by spleen cells

Mukerjee, Ruma ; Misra, Anita ; Chaturvedi, U. C. (1996) Dengue virus-induced cytotoxin releases nitrite by spleen cells International Journal of Experimental Pathology, 77 (2). pp. 45-51. ISSN 0959-9673

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Dengue type-2 virus (DV) infection in mice induces T cells to produce a cytokine, the cytotoxic factor (CF), which induces H2-A positive macrophages to produce another cytokine, cyotoxin (CF2), which amplifies its cytotoxic effects on target cells. The present study was undertaken to investigate the production of nitrite (NO-2) by the spleen cells of mice in vitro and in vivo following inoculation of CF2. Maximum NO-2 production occurred at 1 hour after inoculation of 100 μ g CF2. Pretreatment of CF2 with anti-CF2-antisera (CF2-As) inhibited the production of NO-2. Pretreatment of the spleen cells with NG-monomethyl-l-arginine (NMA) or with arginase inhibited NO-2 production. The NO-2 production was diminished in a dose dependent manner by treatment of spleen cells with the Ca2+ channel blocking drug, nifedipine and Zn2+ as ZnSO4. The findings of the present study thus demonstrate that CF2 induces production of NO-2 in the spleen cells in a CA2+-dependent manner which may be a mechanism of target cell killing.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons, Inc.
Keywords:Dengue Virus; Cytotoxic Cytokine; Nitrite; Cytotoxicity
ID Code:6748
Deposited On:22 Oct 2010 06:26
Last Modified:28 May 2011 08:48

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