Probing the binding interaction of a phenazinium dye with serum transport proteins: a combined fluorometric and circular dichroism study

Bose, Debosreeta ; Sarkar, Deboleena ; Chattopadhyay, Nitin (2010) Probing the binding interaction of a phenazinium dye with serum transport proteins: a combined fluorometric and circular dichroism study Photochemistry and Photobiology, 86 (3). pp. 538-544. ISSN 0031-8655

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1751-...

Related URL: http://dx.doi.org/10.1111/j.1751-1097.2009.00688.x

Abstract

In the present investigation, an attempt has been made to study the interaction of phenosafranin (PSF), a cationic phenazinium dye with the transport proteins, bovine serum albumin (BSA) and human serum albumin (HSA), employing steady-state and time-resolved fluorometric and circular dichroism (CD) techniques. The photophysical properties of the dye are altered on binding with the serum proteins. An explicit study with respect to the modification of the fluorescence and fluorescence anisotropy upon binding, effect of denaturant, fluorescence lifetime and CD measurements reveal that the dye binds to both BSA and HSA with almost the same affinity. Far-UV CD spectra indicate a decrease in the percentage of -helicity only for BSA upon binding with the probe. Near-UV CD responses indicate an alteration in the tertiary structure of both the transport proteins because of binding.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
ID Code:67476
Deposited On:31 Oct 2011 05:15
Last Modified:31 Oct 2011 05:15

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