Production of nitrite by dengue virus-induced cytotoxic factor

Misra, A. ; Mukerjee, Ruma ; Chaturvedi, U. C. (1996) Production of nitrite by dengue virus-induced cytotoxic factor Clinical & Experimental Immunology, 104 (3). pp. 406-411. ISSN 0009-9104

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Dengue type 2 virus (DV) infection induces production of a cytokine, the cytotoxic factor (CF) in the spleen of mice. The present study was undertaken to investigate the production of nitrite (NO-2) by the spleen cells of mice in vitro and in vivo following inoculation of DV or CF. Maximum NO-2 production occurred at 45 min after inoculation of 5 μ g CF, both in vitro and in vivo. The NO-2 was produced by macrophages and T cells and not by B cells. Pretreatment of CF with anti-CF antisera inhibited production of NO-2. DV-stimulated spleen cell culture supernatants showed peak production of CF and NO-2 at 72 h. In DV-infected mouse spleen, maximum NO-2 production occurred at 8-11 days post-infection, which correlated with peak cytotoxic activity in the spleen. Pretreatment of spleen cells with NG-monomethyl l-arginine (NMMA) inhibited NO-2 production. NO-2 production was abrogated in a dose-dependent manner by treatment of spleen cells with Ca2+ channel blocking drug, Nifedipine. The findings demonstrate that DV-induced CF induces production of NO-2 in spleen cells, probably in a Ca2+-dependent manner, and may be a mechanism of target cell killing.

Item Type:Article
Source:Copyright of this article belongs to British Society for Immunology.
Keywords:Dengue Virus; Cytotoxic Factor; Nitrite; Cytotoxicity
ID Code:6741
Deposited On:22 Oct 2010 06:23
Last Modified:16 May 2016 17:02

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