Hepatitis B virus genotypes in chronic liver disease patients from New Delhi, India

Chattopadhyay, Saket ; Das, Bhudev Chandra ; Kar, Premashis (2006) Hepatitis B virus genotypes in chronic liver disease patients from New Delhi, India World Journal of Gastroenterology, 12 (41). pp. 6702-6706. ISSN 1007-9327

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Aim: To study the Hepatitis B virus (HBV) genotypes and their effect on the progression and outcome in patients with chronic liver diseases from New Delhi, India. Methods: Sera from 100 HBV-related chronic liver disease (CLDB) cases were tested for HBV genotype using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and Type-specific primers-based PCR (TSP-PCR) targeting to the surface (S) gene encoding hepatitis B surface antigen. Results: Only genotypes A and D were present and genotype D was dominant. Genotype D was present in all CLDB patient categories. The genotype distribution for the 100 patients with CLDB was as follows: genotype A, 16/100 (16%) (7/40- 17% chronic hepatitis B (CHB); 8/47, 17%, HBV-related cirrhosis (CRB); 1/13, 7.6%, HBV-related hepatocellular carcinoma (HCCB); genotype D- 84/100 (84%) (32/40- 80% CHB; 38/47- 81%, CRB; 11/13, 85%, HCCB); genotype A + D, 3/100 (3%) (1/40- 3% CHB; 1/47- 2%, CRB; 1/13, 7.6%, HCCB); C, 0; B, 0; E, 0; F, 0; G 0, H 0; (P < 0.01, genotype D vs A). Conclusion: Only HBV genotypes A and D were present in patients with CLDB from New Delhi, India. Compared with genotype D, genotype A patients had no signifi cant clinical or biochemical differences (P > 0.05). Mixed infection with genotype A and D were seen in 3% of the cases. Genotype D was the dominant genotype prevalent in all patient categories.

Item Type:Article
Source:Copyright of this article belongs to Wjgnet.
Keywords:HBV-Related Chronic Liver Disease; Hepatitis B Virus Genotypes; PCR-RFLP; Type-Specific Primer-Based PCR
ID Code:66959
Deposited On:07 Nov 2011 04:24
Last Modified:18 May 2016 14:16

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