Diagnostic significance of nested polymerase chain reaction for sensitive detection of Pneumocystis jirovecii in respiratory clinical specimens

Gupta, Rashmi ; Mirdha, Bijay Ranjan ; Guleria, Randeep ; Samantaray, Jyotish Chandra ; Agarwal, Sanjay Kumar ; Kabra, Sushil Kumar ; Luthra, Kalpana (2009) Diagnostic significance of nested polymerase chain reaction for sensitive detection of Pneumocystis jirovecii in respiratory clinical specimens Diagnostic Microbiology and Infectious Disease, 64 (4). pp. 381-388. ISSN 0732-8893

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/j.diagmicrobio.2009.04.008

Abstract

A total of 327 clinical specimens, including both invasive and noninvasive samples, obtained from 275 patients with various types of underlying immunocompromised conditions and a clinical suspicion of Pneumocystis pneumonia (PCP) were subjected to 2 different nested polymerase chain reaction (PCR) assays. The target genes used for nested PCR were mitochondrial large subunit ribosomal RNA (mtLSU rRNA) and internal transcribed spacer (ITS) region. The results were compared with a single-round PCR targeting major surface glycoprotein (MSG) gene. Amplification was successful in 16% of cases by mtLSU rRNA nested PCR, in 14.5% by ITS nested PCR, and in 10.9% by MSG PCR. The nested mtLSU rRNA PCR was found to be more sensitive (100% sensitive and 98.7% specific) and useful in detecting PCP for its use in routine diagnosis in our settings. Thus, this assay may be quite useful in the identification of patients who are in the early stage of Pneumocystis jirovecii infection with an organism load that could not be easily detected by the single-step PCR.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Pneumocystis pneumonia (PCP); Nested PCR; Routine Diagnosis; Invasive and Noninvasive Samples
ID Code:66750
Deposited On:27 Oct 2011 06:42
Last Modified:27 Oct 2011 06:42

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