Mukhopadhyay, R. ; Srivatsan, S. G. ; Verma, S. (2003) Surface trapping and AFM detection of DNA topological intermediates generated from an oxidative chemical nuclease Biochemical and Biophysical Research Communications, 308 (1). pp. 165-169. ISSN 0006-291X
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/S0006-291X(03)01351-2
Abstract
Direct observation of DNA topological intermediates generated from a 'chemical nuclease' treatment has been made by atomic force microscopy (AFM). The intermediates were trapped at the mica-water interface and imaging was carried out in the dynamic force mode. Complete conversion from supercoiled circular state to relaxed circular/linear state has been observed over a time scale of 8 min. Implication of such studies in complementing gel electrophoresis data has been predicted.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | AFM; DNA; Oxidative Cleavage; Chemical Nuclease; Single Molecule |
| ID Code: | 66257 |
| Deposited On: | 21 Oct 2011 09:43 |
| Last Modified: | 21 Oct 2011 09:43 |
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