2-Methyl-pyran-4-one-3-O-β-D-glucopyranoside isolated from leaves of Punica granatum inhibits the TNFα-induced cell adhesion molecules expression by blocking nuclear transcription factor-κB (NF-κB)

Balwani, Sakshi ; Nandi, Debkumar ; Jaisankar, Parasuraman ; Ghosh, Balaram (2011) 2-Methyl-pyran-4-one-3-O-β-D-glucopyranoside isolated from leaves of Punica granatum inhibits the TNFα-induced cell adhesion molecules expression by blocking nuclear transcription factor-κB (NF-κB) Biochimie, 93 (5). pp. 921-930. ISSN 0300-9084

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/j.biochi.2011.01.010

Abstract

Here, we report bioactivity-guided isolation, purification and characterization of a novel compound, 2-methyl-pyran-4-one-3-O-β-D-glucopyranoside (MPG) from the leaves of Punica granatum. The structure of MPG was established on the basis of its detailed spectral analyses. We demonstrated that MPG not only inhibited the expression of cell adhesion molecules but also significantly blocked its functional consequence, that is, the adhesion of neutrophils on human endothelial cells monolayer. To elucidate the molecular mechanism of action of MPG, we showed that MPG decreased the transcript levels of ICAM-1, VCAM-1 and E-selectin genes. Using electrophoretic mobility shift assay (EMSA) and western blot analyses, we demonstrated that MPG significantly blocked both the TNFα-induced translocation and activation of nuclear transcription factor-κB (NF-κB). Thus, MPG could be useful as a novel lead molecule for developing future anti-inflammatory agents.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Punica Granatum; 2-methyl-pyran-4-one-3-O-β-D-glucopyranoside; Tnfα; ICAM-1; VCAM-1; E-selectin; NF-κB
ID Code:66075
Deposited On:21 Oct 2011 03:46
Last Modified:21 Oct 2011 03:46

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